Overview
Microscope

Your global resource for quality chromosome analyses for bioscience research.

Mission Statement
To accelerate the advancement of bioscience research by providing high quality cytogenetic services to investigators worldwide.

Location: Research Triangle Park, North Carolina
With the current widespread use of cultured cells in bioscience research comes the need to confirm that cells being studied are indeed what they are purported to be. Unfortunately, cell cross contamination can and frequently does occur, often unbeknownst to those working most closely with cell cultures. Karyotyping provides an easy solution to verify cell identity. Even in the absence of contamination, many cell lines are in a dynamic state with chromosome complements changing significantly from early to later passage. Changes may occur even when telomerase expression tests are positive, as it takes only a small percentage of cells escaping telomerase expression to produce the fusion-bridge-breakage cycles that drive genomic instability.

Our service diligently works to provide information on extra and missing chromosomes, translocations, deletions, insertions, rings, dicentrics, duplications, double minutes, fragments, markers, breaks, and any other possible aberrations. Whether one is working with novel or established cell lines, data detailing chromosomal constitutions may be of great value.

In 2011, KaryoLogic, Inc. plans to expand services to include fluorescence in situ hybridization and chromosome methylation staining techniques. Please check back at a later date for these services.

Our current focus at KaryoLogic, Inc. is on human, mouse, and rat research specimens. We will gladly attempt other species. Please note that our staff is not certified to diagnose human clinical specimens.

 

Contact Us
Microscope

KaryoLogic, Inc. is located in Research Triangle Park, North Carolina, yet we gladly accept specimens shipped from anywhere around the globe. See "To send a specimen" under "Instructions".

Local customers may arrange for pick-up of specimens from Research Triangle Park, UNC-Chapel Hill, Duke University and other locations in Durham and Orange Counties, North Carolina. A courier will come to your door at no extra charge and transport specimens to KaryoLogic. (This is a limited time offer. See "To arrange for specimen pick-up" under "Instructions".) Members of UNC-Chapel Hill Tissue Culture Facility at Lineberger Comprehensive Cancer Center may drop off specimens at the TCF and will be billed through the TCF. See "Instructions" for further information.

Physical address for shipping:
KaryoLogic, Inc.
601 Keystone Park Drive, Suite 100
Durham, NC 27713

Address for billing, payments, and other correspondence:
KaryoLogic, Inc.
P.O. Box 13505
Research Triangle Park, NC 27709
Email: info@karyologic.com
Phone: (919) 632-6111

 

Staff
Lisa Livanos

Elizabeth "Lisa" Livanos

elivanos@karyologic.com

Education:

B.S. Biological Sciences, Concentration in Genetics, 1983
College of Agriculture and Life Sciences
Cornell University
Ithaca, NY
M.S. Microbiology, Molecular and Cellular Biology Program, 1988
Waksman Institute
Rutgers University
Piscataway, NJ

Ms. Livanos began her career in clinical cytogenetics in the early 1980's. While working at the University of Medicine and Dentistry of NJ in Newark, NJ, and Morristown Memorial Hospital in Morristown, NJ, she gained a thorough knowledge of the human karyotype. After five years in the clinical setting, she moved to North Carolina and began work in research cytogenetics, integrating molecular biology techniques with chromosome analysis. While at UNC-Chapel Hill in the 1990's and early 2000's, Ms. Livanos mastered mouse and rat karyotyping, fluorescence in situ hybridization, sequential banding techniques, and comparative genomic hybridization. From 2004 to 2006, she expanded her skills by learning a variety of genetic toxicology assays at Integrated Laboratory Systems, Inc. in Research Triangle Park, NC. Gaining much satisfaction from consulting work for such institutions as National Institute of Environmental and Health Sciences, and Glaxo Smith Kline, among others, Ms. Livanos decided to launch KaryoLogic, Inc. in 2008 to bring valuable cytogenetic techniques within easier reach of the greater research community.

Relevant Publications

Fulcher ML, Gabriel SE, Olsen JC, Tatreau JR, Gentzsch M, Livanos E, Saavedra MT, Salmon P, and Randell SH. Novel human bronchial epithelial cell lines for cystic fibrosis research. Am J Physiol Lung Cell Mol Physiol. 296 (1): L82-91, 2009.

Simpson DA, Livanos E, Heffernan TP, and Kaufmann WK. Telomerase expression is sufficient for chromosomal integrity in cells lacking p53 dependent G1 checkpoint function. J. Carcinog. 4:18-34, 2005.

Slebos RJ, Livanos E, Yim HW, Randell SH, Parsons AM, Detterbeck FC, Rivera MP, and Taylor JA. Chromosomal abnormalities in bronchial epithelium from smokers, non-smokers, and lung cancer patients. Cancer Genet Cytogenet. 159 (2): 137-142, 2005.

Xiong Y, Kuppuswamy D, Li Y, Livanos EM, Hixon M, White A, Beach D, and Tlsty TD. Alteration of cell cycle kinase complexes in human papillomavirus E6-and E7- expressing fibroblasts precedes neoplastic transformation. J Virol. 70: 999-1008, 1996.

Coleman WB, McCullough KD, Esch GL, Civalier CJ, Livanos E, Weissman BE, Grisham JW, and Smith GJ. Suppression of tumorigenic phenotype of a rat liver epithelial tumor cell line by the p11.2-p12 region of human chromosome 11. Mol Carcinog. 13: 220-232, 1995.

White AE, Livanos EM, and Tlsty TD. Differential disruption of genomic integrity and cell cycle regulation in normal human fibroblasts by the HPV oncoproteins. Genes & Devel. 8: 666-677, 1994.

Witsell DL, Weissler MC, Livanos E, Bova-Hill C, and Gilmer TM. Unique chromosomal rearrangement and mucin production in a novel salivary myoepithelial cell strain. Arch Otolaryngol Head Neck Surg. 119: 1151-1157, 1993.

Schaefer DI, Livanos EM, White AE, and Tlsty TD. Multiple mechanisms of N-(phosophonoacetyl)-L-aspartate drug resistance in SV40-infected precrisis human fibroblasts. Cancer Res. 53: 4946-4951, 1993.

Livingstone LR, White A, Sprouse J, Livanos E, Jacks T, and Tlsty TD. Altered cell cycle arrest and gene amplification potential accompany loss of wild-type p53. Cell. 70: 923-935, 1992.

Loh WE, Scrable HJ, Livanos E, Arboleda MJ, Cavenee WK, Oshimura M and Weissman BE. Human chromosome 11 contains two different growth suppressor genes for embryonal rhabdomyosarcoma. Proc Natl Acad Sci USA. 89: 1755-1759, 1992.

Dowdy SF, Fasching CL, Araujo D, Lai KM, Livanos E, Weissman BE and Stanbridge EJ. Suppression of tumorigenicity in Wilms tumor by the p15.5-p14 region of chromosome 11. Science 254: 293-295, 1991.

 

Services

Simple G-band Analysis

Recommended for answering simple, straightforward questions, e.g., Is there cell cross contamination in this culture? Are these cells male or female? What specie is this culture? Fee covers receipt of live cells, brief culture, harvest, fixation, slidemaking, banding/staining of chromosomes, and microscope analysis. A minimum of 5 metaphase spreads will be analyzed, not to exceed 1 hour of microscopy time. A minimum of 1 image will be provided along with a count sheet detailing chromosomal observations. At the request of the client, this analysis can be extended to become the more detailed "Standard Analysis" if something of interest is discovered.

Standard G-band Analysis

Recommended for gathering typical information on chromosome constitutions such as chromosome number, trisomies, monosomies, deletions, insertions, translocations, dicentrics, rings, breaks, double minutes, etc., for cells that are diploid or near diploid. Fee covers receipt of live cells, brief culture, harvest, fixation, slidemaking, banding/staining of chromosomes, and microscope analysis. A minimum of 20 metaphase spreads will be analyzed, not to exceed 3 hours of microscopy time. A minimum of 2 karyotypes will be provided along with a count sheet detailing chromosomal observations. This analysis may be charged at the rate of the "Complex Analysis" if more microscopy time is required. At the request of the client, this analysis can be extended to become a more detailed "Complex Analysis" if something of interest is discovered.

Complex G-band Analysis

Recommended for cells with highly rearranged karyotypes and a wide variance of chromosome count per cell OR for cells with less rearranged, near diploid karyotypes for which the client desires up to 50 metaphases analyzed. Fee covers receipt of live cells, brief culture, harvest, fixation, slidemaking, banding/staining of chromosomes, and microscope analysis. A minimum of 25 metaphase spreads will be analyzed for cells with highly rearranged karyotypes. A minimum of 3 karyotypes will be provided along with a count sheet detailing chromosomal observations.

Karyotyping of Mouse Embryonic Stem Cells Before Injection

Fee covers receipt of live cells, brief culture, harvest, fixation, slidemaking, banding/staining of chromosomes, and microscope analysis. A minimum of 50 metaphase spreads will be counted and scored for gross chromosome aberrations. A minimum of 1 image will be provided along with a count sheet detailing chromosomal observations.

For all of the above services:

  • Work will be performed in the spirit of GLP (Good Laboratory Practice).
  • Expect an average turn around time of two weeks.
  • A rush order may be placed for an extra fee.
  • An invoice will be sent after completed work is delivered (either by email or postal service), with payment due 30 days from invoice date.

To view our price list, click Fees, below.

 

Fees

Pricing for Academic and Government Institutions

Human
Simple $300
Standard $550
Complex $750
Mouse and Rat
Simple $300
Standard $650
Complex $850
Mouse ES cells for injection $375
Other fees
Rush Order Add an extra $75
Karyotyping of other species Please inquire by email: info@karyologic.com
Failure to get any result 10% of anticipated fee
Failure to complete an analysis Fee pro-rated dependent upon percent completed

Pricing for Private Industry

Human
Simple $350
Standard $650
Complex $850
Mouse and Rat
Simple $350
Standard $750
Complex $950
Mouse ES cells for injection $425
Other fees
Rush Order Add an extra $100
Karyotyping of other species Please inquire by email: info@karyologic.com
Failure to get any result 10% of anticipated fee
Failure to complete an analysis Fee pro-rated dependent upon percent completed

 

To send a specimen

Shipment

Prepare two to three 25cm2 flasks with cells at varying confluencies, (e.g. 20%, 50%, and 80%) with a minimum of 500,000 cells seeded per flask, for each cell line. Make sure flasks are clearly labeled. Fill each flask to the top with media. Tighten caps and wrap well with parafilm around the necks and caps of the flasks. (Some vented caps may leak, though most brands hold up well.) Place inside a secondary container like a sealed bag or plastic canister with enough absorbent material to soak up the media in the case of breakage. Pack in a third outer container. Ship overnight, if possible, at ambient temperature. Enclose contact information and any special instructions. If you believe there is minimal likelihood that pathogens are present in your samples, merely triple package as described and label the outside of the container‚ "Exempt Human Specimens", or "Exempt Animal Specimens".

However, for samples which are known, or are reasonably expected, to contain pathogens (but not capable of causing permanent disability or life threatening disease to humans or animals) then, in accordance with IATA and 49 CFR regulations, your specimens fall under the restrictions of UN 3373 Category B. Carriers will require additional specifications in packaging, labeling, and shipment.

In addition, click on the Order Form below to send specimen and billing information, preferably 24 hours before cells are due to arrive.

Shipping Address:
KaryoLogic, Inc.
601 Keystone Park Drive, Suite 100
Durham, NC 27713

Drop off

Prepare two to three 25cm2 flasks with cells at varying confluencies, (e.g. 20%, 50%, and 80%) with a minimum of 500,000 cells seeded per flask, for each cell line. Make sure flasks are clearly labeled. Use the Order Form below to send specimen and billing information, preferably 24 hours before cells are due to arrive. Bring specimens to 601 Keystone Park Drive, Suite 100, Durham, NC 27713.

Directions

  • Take I-40 to Exit 280 ‚ Davis Drive
  • At the end of the exit ramp, make a left onto Davis Drive.
  • Travel approximately 1.5 miles and make a left onto Park Knoll Drive.
  • Go to the STOP sign. Make a right onto Keystone Park Drive.
  • Travel approximately 50 yards and make a left into the parking lot for Building 601.
  • Go to the back of the building and bring the specimens to the door of Suite 100. ('ILS' is written over the doorway.)
  • Ring doorbell. Call (919) 632-6111 with questions or problems.

 

To arrange for specimen pickup

UNC-Chapel Hill, Chapel Hill, NC

Customers at UNC-Chapel Hill may opt to drop off specimens at the UNC Lineberger Comprehensive Cancer Center Tissue Culture Facility. Prepare two to three 25cm2 flasks with cells at varying confluencies, (e.g. 20%, 50%, and 80%) with a minimum of 500,000 cells seeded per flask, for each cell line. Make sure flasks are clearly labeled. Click on the Order Form below to send specimen information. Bring flasks to the Tissue Culture Facility on the third floor of the Lineberger Comprehensive Cancer Center. Billing will be through the TCF.

Other locations in Durham and Orange Counties, NC

Prepare two to three 25cm2 flasks with cells at varying confluencies, (e.g. 20%, 50%, and 80%) with a minimum of 500,000 cells seeded per flask, for each cell line. Make sure flasks are clearly labeled. Use the Order Form below to send specimen and billing information, being sure to request specimen pick-up. Someone will contact you as soon as the email is received to arrange details of when and where to hand off the cells.

Order Form

Contact Information

Fields with asterisks (*) are required.
Lab Director
Company/University*
Department
Contact Name*

Address to send results:

Address 1*
Address 2
City*
State/Province*
Postal/Zip code*
Country*
Telephone*
Email address*

For customers dropping off cells at the UNC Lineberger CCC Tissue Culture Facility in Chapel Hill, skip to cell information section. You will be billed through the TCF after work is completed.
Billing address same as above?



Company/University
Address 1
Address 2
City
State/Province
Postal/Zip code
Country
Purchase Order #
Invoice will be sent after report is completed and delivered. Payment is due 30 days from invoice date. For foreign clients, currency conversion will be calculated at the time of billing.

Transport Information

*Transport method

facility
Specimen pick-up by KaryoLogic courier. (For customers in Durham and Orange Counties, NC. You will be contacted shortly to make arrangements.)

Drop-off date (if drop-off)
Drop-off time (if drop-off)
Shipment arrival date (if shipped)

Cell Information (Sample 1)

Cell Name
Specie
Passage/Population Doubling Number
Approximate Doubling Time
Type of Analysis

         

Special instructions

Cell Information (Sample 2)

Cell Name
Specie
Passage/Population Doubling Number
Approximate Doubling Time
Type of Analysis               
Special instructions

Cell Information (Sample 3)

Cell Name
Specie
Passage/Population Doubling Number
Approximate Doubling Time
Type of Analysis               
Special instructions


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